Osmosis LabBackground information:Osmosis is the movement of water down a concentration gradient across a semipermeable membrane. In this lab,you will be observing what happens to a cell model (a raw egg without its shell) when it is in 2 different solutions.One solution (the hypotonic solution) will contain a higher concentration of water molecules relative to the amountof water molecules inside of the egg. The other solution (hypertonic) will contain a lower concentration of watermolecules relative to the amount of water molecules inside of the egg.Materials:2 raw eggs,2 small clear containers that are big enough submerge an egg in,1 medium clear container that is big enough to submerge 2 eggs in,enough vinegar to completely submerge 2 eggs in your medium size container,enough water to completely submerge an egg in your small container,enough Kyro (or other brand of) corn syrup to completely submerge an egg in your small container.A ruler for measuring the approximate diameter of your eggsEnough foil, plastic-wrap or other material to cover your 2 small clear containersNOTE: Since raw eggs may contain Salmonella, wash your hands with soap and water after handling raw eggs.Procedure:1) Dissolve the eggshell of 2 eggs by submerging the eggs in vinegar for 2 days. In the observation section below,summarize what you observed happening in the container while the eggshell dissolved.2) After the eggshell has dissolved, while being careful not to break the exposed membrane, remove both eggs fromthe vinegar and rinse them with water. Gently place one egg in each of the 2 small clear containers. In theobservation section below, record the approximate diameter of each egg.3) Into one of the small containers, pour enough water to completely submerge one of your shell-less eggs. Coverthe container to prevent the water from evaporating. In the observation section below, record the time and date thatyou submerged this egg in water.4) Into the other small container, pour enough corn syrup to completely submerge one of your shell-less eggs.Cover the container to prevent the corn syrup from drying out. In the observation section below, record the time anddate that you submerged this egg in corn syrup.5) After about 24 hours, measure the approximate diameters of each of your submerged eggs. In the observationsection below, record your measurements as well as the time and date that you took them.6) You should see a noticeable difference in the sizes of both eggs relative to each other and the size they started at.If you don’t see a noticeable size difference, leave the eggs in their solutions for about another 12 hours and thenmeasure them, again.7) Put the eggs in the garbage, wash the vinegar and corn syrup down the sink, and throw away your threecontainers (or wash them well if they are reusable).Observations:(5 points) Observation of dissolving eggshell:(5 points) Approximate diameter of egg you will put in water:(5 points) Approximate diameter of egg you will put in corn syrup:(5 points) Time and date that you submerged shell-less egg in water:(5 points) Time and date that you submerged shell-less eggs in corn syrup:(5 points) Measurement of egg that has been submerged in water for about 24 hours: Time & date:(5 points) Measurement of egg that has been submerged in corn syrup for about 24 hours: Time & date:Analysis and conclusion:1) (5 points) What were the independent variables in this experiment?2) (5 points) What were the dependent variables in this experiment?3) (5 points) Describe the materials you used.4) (20 points) In your own words, describe the procedures you followed.5) (10 points) Summarize the results of submerging a shell-less egg in water compared to corn syrup.6) (5 points) Based on your observations, which is the hypertonic solution, and which is the hypotonic solution?7) (5 points) Base your answer to the following question on what you know about the concentrations of watermolecules in water and corn syrup compared to the concentration of water molecules in the egg. Which is thehypertonic solution, and which is the hypotonic solution?8) (5 points) What, if anything, didn’t go as smoothly as planned? And/or what would you do differently if you didthis activity again?9) (5 points) What questions or insights did this activity or your results raise for you? And/or describe yourreactions to doing this assignment.
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